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超敏ECL化學發光液

產品編號 產品名稱 規格 價格(元) 說明書
M2301 超敏ECL化學發光液 50ml/500ml 300/2,000
Western Blot結果的好壞與否與ECL發光液的選擇有著密切關系,好的ECL發光液不僅可曝光得到漂亮的目的條帶,還可節省昂貴的抗體。海基超敏ECL發光液用于Western、Northern和Southern blot分子雜交的化學發光檢測,原理是采用新型發光底物(Luminol),其與辣根過氧化物酶反應時產生很強的發光信號,以此檢測微量蛋白而獲得理想的實驗結果。比傳統化學顯色法靈敏度高,可達pg水平,發光持續時間長,信號30min內不會發生明顯降低。 超敏ECL發光液

組分

組分名稱 數量 保存
Super ECL A 25 ml 4℃
Super ECL B 25 ml 4℃

應用

在Western Blot印跡分析,核酸雜交以及EMSA實驗中,HRP標記的二抗或探針與靶分子結合再與底物反應產生可見光。

儲存

2-8°C,Super ECL A要求避光保存。

實例

超敏ECL發光液   ECL發光液
Fig1.檢測Hela細胞裂解物的GAPDH蛋白,應用超敏ECL發光液(HaiGene,M2301)顯色,一抗為兔抗GAPDH多克隆抗體,稀釋比例為1:1000。   Fig2.檢測A549細胞裂解物的AKT蛋白,應用超敏ECL發光液(HaiGene,M2301)和Competitor P公司的ECL發光液分別顯色,
一抗為AKT rabbit mab(CST,#4691),稀釋比例為1:1000。

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